英文题目Cognitive rigidity and BDNF-mediated frontostriatal glutamate neuroadaptations during spontaneous nicotine withdrawal.


作者Cole RD

作者单位:Department of Psychology and Neuroscience Program, Temple University,

Philadelphia, PA, 19122, USA国宾夕法尼亚州费城坦普尔大学心理学和神经科学系

期刊Neuropsychopharmacology.   发表时间:2019-11     IF6.22

DOI: 10.1038/s41386-019-0574-6



Cognitive flexibility is the ability to switch strategic responses adaptively in  changing environments. Cognitive rigidity imposed by neural circuit adaptations during nicotine abstinence may foster maladaptive nicotine taking in addicts. We  systematically examined the effects of spontaneous withdrawal in mice exposed to  either nicotine (6.3 or 18 mg/kg/day) or saline for 14 days on cognitive flexibility using an operant strategy set-shifting task. Because frontostriatal circuits are critical for cognitive flexibility and brain-derived neurotrophic factor (BDNF) modulates glutamate plasticity in these circuits, we also explored  the effects of nicotine withdrawal on these neurochemical substrates. Mice undergoing nicotine withdrawal required more trials to attain strategy-switching  criterion. Error analysis show that animals withdrawn from both nicotine doses committed higher perseverative errors, which correlated with measures of anxiety.

However, animals treated with the higher nicotine dose also displayed more strategy maintenance errors that remained independent of negative affect. BDNF mRNA expression increased in the medial prefrontal cortex (mPFC)  followingnicotine withdrawal. Surprisingly, BDNF protein declined in mPFC but was elevated in dorsal striatum (DS). DS BDNF protein positively correlated with perseverative and maintenance errors, suggesting mPFC-DS overflow of BDNF during withdrawal. BDNF-evoked glutamate release and synapsin phosphorylation was attenuated within  DS synapses, but enhanced in the nucleus accumbens, suggesting a dichotomous role

of BDNF signaling in striatal regions. Taken together, these data suggest that spontaneous nicotine withdrawal impairs distinct components of cognitive set-shifting and these deficits may be linked to BDNF-mediated alterations in glutamate signaling dynamics in discrete frontostriatal circuits.





英文题目:N-WASP knockdown upregulates inflammatory cytokines expression in human gingival  fibroblasts.


作者:Wang Y

单位:Department of Periodontology, School and Hospital of Stomatology, Shandong

University 山东省口腔医学院牙周病学系

期刊:ARCHIVES OF ORAL BIOLOGY  发表时间:2019-11  IF1.88

DOI: 10.1016/j.archoralbio.2019.104605



OBJECTIVE: The neuronal wiskott-aldrich syndrome protein (N-WASP) is a member of  the wiskott-aldrich syndrome protein (WASP) family. N-WASP plays a vital role in  promoting cell migration, receptor signaling and immune inflammatory responses. This study aimed to observe the changes in the expression of inflammatory factors and involving pathways after N-WASP knockdown in human gingival fibroblasts (HGFs).

DESIGN: Gingival inflammatory condition of N-WASP knockout mice was evaluated by  H&E staining. N-WASP in HGFs was knockdown by siRNA and the best knockdown efficiency was determined by qRT-PCR and immunofluorescence. The mRNA levels of interleukin (IL)-6, IL-8, C-C motif ligand 2 (CCL2), superoxide dismutase 2 (SOD2) and prostaglandin endoperoxide synthase 2 (PTGS2) were evaluated by qRT-PCR after N-WASP knockdown with or without mitogen-activated protein kinase (MAPK) and nuclear factor-κB (NF-κB) inhibitors. The protein levels of IL-6, IL-8 and CCL2 were assessed by ELISA. Western blotting was used to detect the activation of NF-κB and MAPK signaling pathways.

RESULTS: Gingival tissue from N-WASP knockout mice exhibited an inflammatory reaction. The expression of IL-6, IL-8, CCL2, SOD2 and PTGS2 was significantly upregulated after N-WASP knockdown in HGFs for 6, 24 and 48 h, except for the SOD2 at 6 h. N-WASP knockdown significantly activated the signaling pathways of NF-κB and MAPK. The inhibitors of p65, p38, ERK and JNK clearly decreased IL-6, IL-8, CCL2, SOD2 and PTGS2 expression after N-WASP knockdown. CONCLUSION: These data indicated that N-WASP deficiency in HGFs increases the production of inflammatory cytokine and is regulated via NF-κB and MAPK  signaling pathways.


目的:神经元wiskott-aldrich综合征蛋白(N-WASP)是wiskott-aldrich综合征蛋白(WASP)家族的一员。黄蜂在促进细胞迁移,受体信号与免疫炎症反应本研究旨在观察N-WASP基因敲除人牙龈成纤维细胞(HGFs)后炎症因子表达及相关通路的变化。设计:采用H&E染色法评价N-WASP基因敲除小鼠牙龈炎症状况。用siRNA基因敲除HGFs中的N-黄蜂,并用qRT-PCR和免疫荧光检测其效率。白细胞介素(IL- 6IL-8C-C基序配体2CL2)、超氧化物歧化酶2SOD2)和前列腺素内过氧化物合酶2PGGS2)的mRNA水平在Q-RT-PCR检测N-WASP敲除或不含丝裂原活化蛋白激酶(MAPK)和核因子-κBNF-κB)抑制剂后的表达水平。ELISA法检测IL-6IL-8CCL2蛋白水平。结果:N-WASP基因敲除小鼠牙龈组织呈炎症反应。HGFsN-WASP基因敲除62448小时后,IL-6IL-8CCL2SOD2PTGS2的表达显著上调,但6小时的SOD2基因敲除明显激活了NF-κBMAPK的信号通路。p65p38ERKJNK抑制剂明显降低N-WASP基因敲除后IL-6IL-8CCL2SOD2PTGS2的表达。结论:HGFsN-WASP的缺失增加了炎性细胞因子的产生,并通过NF-κBMAPK信号途径进行调控。



英文题目:Novel BDNF-regulatory microRNAs in neurodegenerative disorders pathogenesis: An in silico study.


作者:Khani-Habibabadi F

单位:Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares

University, Tehran, Iran.伊朗德黑兰大学塔比亚特莫代雷斯生物科学学院遗传学系


DOI: 10.1016/j.compbiolchem.2019.107153



Brain-derived neurotrophic factor (BDNF) is a neurotrophic factor with various roles in the central nervous system neurogenesis, neuroprotection, and axonal guide. By attaching to Tropomyosin receptor kinase B (TrkB) receptor, this protein triggers downstream signaling pathways which lead to cellular growth, proliferation, survival, and neuroplasticity. Deregulation at mRNA level is involved in various central nervous system disorders including, Huntington, Alzheimer\'s, Multiple Sclerosis, and Amyotrophic Lateral Sclerosis diseases. Considering the importance of BDNF functions, deciphering the regulatory mechanisms controlling BDNF expression level could pave the way to develop more accurate and efficient treatments for neurological diseases. Among different regulatory systems, microRNAs (miRNAs) play prominent roles by targeting genes 3\' untranslated regions. In this study, 127 validated and bioinformatic-predicted miRNAs with potentially regulatory roles in BDNF expression were analyzed.  Various aspects of miRNAsö possible functions were assessed by bioinformatic online tools to find their potential regulatory functions in signaling pathways,  neurological disorders, expression of transcription factors and miRNAs sponge. Analyzed data led to introduce 5 newly reported miRNAs that could regulate BDNF  expression level. Finally, high throughput sequencing data from different brain regions and neurological disorders were analyzed to measure correlation of candidate miRNAs with BDNF level in experimental studies. In this study, a list of novel miRNAs with possible regulatory roles in BDNF expression level involving in different neurological disorders was introduced.





英文题目:Evaluation of a co-extraction kit for mRNA, miRNA and DNA methylation-based body  fluid identification.


作者:Watanabe K

单位:National Research Institute of Police Science, Chiba 277-0882, Japan. Electronic address: k-watanabe@nrips.go.jp.(国立警察科学研究所)

期刊:Leg Med      发表时间:2019-11       IF0.152

DOI: 10.1016/j.legalmed.2019.101630



Recently, messenger RNA (mRNA), micro RNA (miRNA), and DNA methylation (DNAm) have been reported as novel markers for body fluid identification (BFID). Comprehensive analysis of these markers should be a flexible and reliable BFID method for various types of forensic samples. However, independent extraction of  all targets can be difficult depending on the usable amounts of samples. In this  study, the applicability of a co-extraction kit for these molecules, the AllPrep DNA/RNA/miRNA Universal Kit (APU), was evaluated by comparing RNA and DNA extracted from blood and saliva stains by the APU with those extracted by standard kits for each molecule and by previously reported methods for mRNA/DNA or miRNA/DNA co-extraction. Electrophoresis using the Bioanalyzer platform and real-time PCR analysis revealed that the APU performed almost equivalently to each standard kit in the quality of RNA or DNA extracted and extraction efficiency of mRNAs, miRNAs, and DNA. Moreover, the APU outperformed the co-extraction methods, especially in RNA integrity and miRNA extraction efficiency. In addition, pyrosequencing revealed that the methylation ratios of DNA extracted by the APU were not different from those extracted by standard DNA  extraction kits. Overall, the APU is applicable to comprehensive analysis of mRNA/miRNA/DNAm markers for BFID analysis. Because the DNA eluate can also be used for DNA typing, the APU may be among the best choices for forensic examination of body fluid samples in terms of its flexibility and reliability in  BFID and efficiency in sample consumption.





英文题目:Label-Free proteomic analysis reveals the differentiation between unfertilized and fertilized Beijing-You chicken eggs.


作者:Zhang L

单位:Institute of Food Science and Technology, Chinese Academy of Agricultural

Sciences, Beijing 100193, PR China.中国农业科学院食品科学技术研究所

期刊:Int J Biol Macromol. 发表时间:2019-11    IF 2.858

DOI: 10.1016/j.ijbiomac.2019.10.189



Egg fertilization is a dynamic process, including varieties of biochemical changes. To better understand the molecular mechanisms during the egg embryo development, the objective of this study was to quantify protein expression changes between fertilized and unfertilized Beijing-You chicken eggs using label-free liquid chromatography-tandem mass spectrometry method. The results showed that a total of 1241 proteins were identified from fertilized and unfertilized eggs, 229 proteins were observed difference in fertilized eggs (p<0.05) compared with that in unfertilized eggs. The expressions of 86 proteins  were up-regulated and 48 proteins were down-regulated in fertilized eggs. STRING database analysis and Gene Ontology analysis results showed that these differentially expressed proteins significantly interacted and were involved in lipid transport and inflammatory response biological processes. The mRNA and protein expression levels of most differentially expressed proteins Apolipoprotein B, Fibrinogen alpha chain, Transferrin receptor protein 1, Phospholipid transfer protein and Vimentin were validated by RT-PCR and western blot. These results could provide possible novel insights for the molecular mechanism of egg fertilization.




Hello ATCG 2019-11-27 18:01:15